LOCUS       40924_5264       11889 bp DNA     circular SYN 17-DEC-2018
DEFINITION  Gateway vector pB4GWcY DNA, complete sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11889)
  AUTHORS   Kamigaki A, Nito K, Hikino K, Goto-Yamada S, Nishimura M, Nakagawa 
            T, Mano S.
  TITLE     Gateway Vectors for Simultaneous Detection of Multiple 
            Protein-Protein Interactions in Plant Cells Using Bimolecular 
            Fluorescence Complementation
  JOURNAL   PLoS ONE 11 (8), E0160717 (2016)
  PUBMED    27490375
REFERENCE   2  (bases 1 to 11889)
  AUTHORS   Mano S, Nakagawa T.
  TITLE     Direct Submission
  JOURNAL   Submitted (10-JUL-2013) Contact:Shoji Mano National Institute for 
            Basic Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji,
            Okazaki, Aichi 444-8585, Japan
REFERENCE   3  (bases 1 to 11889)
  TITLE     Direct Submission
REFERENCE   4  (bases 1 to 11889)
  AUTHORS   .
  TITLE     Direct Submission
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS ONE"; 
            date: "2016"; volume: "11"; issue: "8"; pages: "E0160717"
COMMENT     SGRef: number: 2; type: "Journal Article"; journalName: "Submitted 
            (10-JUL-2013) Contact:Shoji Mano National Institute for Basic 
            Biology, Department of Cell Biology; 38 Nishigonaka, Myodaiji, 
            Okazaki, Aichi 444-8585, Japan"
COMMENT     SGRef: number: 3; type: "Journal Article"
COMMENT     These colnes were obtained at our laboratory.
FEATURES             Location/Qualifiers
     source          1..11889
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    complement(114..138)
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     primer_bind     341..357
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        872..1217
                     /label=CaMV 35S promoter
                     /note="strong constitutive promoter from cauliflower mosaic
                     virus"
     protein_bind    1239..1363
                     /label=attR1
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        1400..1430
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an 'up' mutation"
     CDS             1484..2140
                     /codon_start=1
                     /label=CmR
                     /note="chloramphenicol acetyltransferase"
                     /translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
                     KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
                     LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
                     DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
     CDS             2485..2787
                     /codon_start=1
                     /label=ccdB
                     /note="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     protein_bind    complement(2831..2955)
                     /label=attR2
                     /note="recombination site for the Gateway(R) LR reaction"
     CDS             2966..3166
                     /codon_start=1
                     /product="carboxy-terminal fragment of enhanced yellow
                     fluorescent protein"
                     /label=carboxy-terminal fragment of enhanced yellow fl
                     /note="ceyfp"
                     /protein_id="BAV44513.1"
                     /translation="MGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDH
                     MVLLEFVTAAGITLGMDELYK"
     terminator      3188..3440
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     primer_bind     complement(3474..3490)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3498..3514
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(3522..3552)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    complement(3567..3588)
                     /label=CAP binding site
                     /note="CAP binding activates transcription in the presence
                     of cAMP."
     terminator      complement(3622..3874)
                     /label=NOS terminator
                     /note="nopaline synthase terminator and poly(A) signal"
     CDS             complement(4267..5055)
                     /codon_start=1
                     /label=NeoR/KanR
                     /note="aminoglycoside phosphotransferase from Tn5"
                     /translation="IEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRPV
                     LFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLSS
                     HLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQG
                     LAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIAL
                     ATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     promoter        complement(5079..5262)
                     /label=NOS promoter
                     /note="nopaline synthase promoter"
     misc_feature    complement(5468..5492)
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA"
     CDS             6020..6808
                     /codon_start=1
                     /label=SmR
                     /note="aminoglycoside adenylyltransferase (Murphy, 1985)"
                     /translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      7055..7643
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     misc_feature    complement(7829..7969)
                     /label=bom
                     /note="basis of mobility region from pBR322"
     rep_origin      complement(8313..8507)
                     /direction=LEFT
                     /label=pVS1 oriV
                     /note="origin of replication for the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
     CDS             complement(8576..9640)
                     /codon_start=1
                     /label=pVS1 RepA
                     /note="replication protein from the Pseudomonas plasmid
                     pVS1 (Heeb et al., 2000)"
                     /translation="GRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESWQA
                     AADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLSKR
                     DRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKPGR
                     VFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGEAL
                     ISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYRLA
                     RRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPILV
                     MRYRNLIEGEASAGS"
     CDS             complement(10077..10703)
                     /codon_start=1
                     /label=pVS1 StaA
                     /note="stability protein from the Pseudomonas plasmid pVS1
                     (Heeb et al., 2000)"
                     /translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
                     DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
                     VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
                     LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
        1 ttgatcccga ggggaaccct gtggttggca tgcacataca aatggacgaa cggataaacc
       61 ttttcacgcc cttttaaata tccgttattc taataaacgc tcttttctct taggtttacc
      121 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
      181 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
      241 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
      301 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
      361 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
      421 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
      481 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
      541 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
      601 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
      661 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
      721 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
      781 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
      841 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
      901 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
      961 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
     1021 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
     1081 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
     1141 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
     1201 ttcatttgga gagaacacgg gggactctag agttatcaac aagtttgtac aaaaaagctg
     1261 aacgagaaac gtaaaatgat ataaatatca atatattaaa ttagattttg cataaaaaac
     1321 agactacata atactgtaaa acacaacata tccagtcact atggcggccc ctcgaggggc
     1381 cgcattaggc accccaggct ttacacttta tgcttccggc tcgtataatg tgtggatttt
     1441 gagttaggat ccggcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac
     1501 tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca
     1561 gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa
     1621 gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct
     1681 gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg tgatatggga
     1741 tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg
     1801 gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg
     1861 ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt ttttcgtctc
     1921 agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata tggacaactt
     1981 cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg tgctgatgcc
     2041 gctggcgatt caggttcatc atgccgtttg tgatggcttc catgtcggca gaatgcttaa
     2101 tgaattacaa cagtactgcg atgagtggca gggcggggcg taaagatctg gatccggctt
     2161 actaaaagcc agataacagt atgcgtattt gcgcgctgat ttttgcggta taagaatata
     2221 tactgatatg tatacccgaa gtatgtcaaa aagaggtgtg ctatgaagca gcgtattaca
     2281 gtgacagttg acagcgacag ctatcagttg ctcaaggcat atatgatgtc aatatctccg
     2341 gtctggtaag cacaaccatg cagaatgaag cccgtcgtct gcgtgccgaa cgctggaaag
     2401 cggaaaatca ggaagggatg gctgaggtcg cccggtttat tgaaatgaac ggctcttttg
     2461 ctgacgagaa caggggctgg tgaaatgcag tttaaggttt acacctataa aagagagagc
     2521 cgttatcgtc tgtttgtgga tgtacagagt gatattattg acacgcccgg gcgacggatg
     2581 gtgatccccc tggccagtgc acgtctgctg tcagataaag tctcccgtga actttacccg
     2641 gtggtgcata tcggggatga aagctggcgc atgatgacca ccgatatggc cagtgtgccg
     2701 gtctccgtta tcggggaaga agtggctgat ctcagccacc gcgaaaatga catcaaaaac
     2761 gccattaacc tgatgttctg gggaatataa atgtcaggct cccttataca cagccagtct
     2821 gcaggtcgac catagtgact ggatatgttg tgttttacag tattatgtag tctgtttttt
     2881 atgcaaaatc taatttaata tattgatatt tatatcattt tacgtttctc gttcagcttt
     2941 cttgtacaaa gtggttgata acagcatggg cagcgtgcag ctcgccgacc actaccagca
     3001 gaacaccccc atcggcgacg gccccgtgct gctgcccgac aaccactacc tgagctacca
     3061 gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt
     3121 gaccgccgcc gggatcactc tcggcatgga cgagctgtac aagtaagctt agagctcgaa
     3181 tttccccgat cgttcaaaca tttggcaata aagtttctta agattgaatc ctgttgccgg
     3241 tcttgcgatg attatcatat aatttctgtt gaattacgtt aagcatgtaa taattaacat
     3301 gtaatgcatg acgttattta tgagatgggt ttttatgatt agagtcccgc aattatacat
     3361 ttaatacgcg atagaaaaca aaatatagcg cgcaaactag gataaattat cgcgcgcggt
     3421 gtcatctatg ttactagatc gggaattggt tccggaacca attcgtaatc atggtcatag
     3481 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
     3541 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattagg ctgaattagg
     3601 cgcgcctatt tctgaattcc cgatctagta acatagatga caccgcgcgc gataatttat
     3661 cctagtttgc gcgctatatt ttgttttcta tcgcgtatta aatgtataat tgcgggactc
     3721 taatcataaa aacccatctc ataaataacg tcatgcatta catgttaatt attacatgct
     3781 taacgtaatt caacagaaat tatatgataa tcatcgcaag accggcaaca ggattcaatc
     3841 ttaagaaact ttattgccaa atgtttgaac gatcggggat catccgggtc tgtggcggga
     3901 actccacgaa aatatccgaa cgcagcaaga tatcgcggtg catctcggtc ttgcctgggc
     3961 agtcgccgcc gacgccgttg atgtggacgc cgggcccgat catattgtcg ctcaggatcg
     4021 tggcgttgtg cttgtcggcc gttgctgtcg taatgatatc ggcaccttcg accgcctgtt
     4081 ccgcagagat cccgtgggcg aagaactcca gcatgagatc cccgcgctgg aggatcatcc
     4141 agccggcgtc ccggaaaacg attccgaagc ccaacctttc atagaaggcg gcggtggaat
     4201 cgaaatctcg tgatggcagg ttgggcgtcg cttggtcggt catttcgaac cccagagtcc
     4261 cgctcagaag aactcgtcaa gaaggcgata gaaggcgatg cgctgcgaat cgggagcggc
     4321 gataccgtaa agcacgagga agcggtcagc ccattcgccg ccaagctctt cagcaatatc
     4381 acgggtagcc aacgctatgt cctgatagcg gtccgccaca cccagccggc cacagtcgat
     4441 gaatccagaa aagcggccat tttccaccat gatattcggc aagcaggcat cgccatgggt
     4501 cacgacgaga tcatcgccgt cgggcatgcg cgccttgagc ctggcgaaca gttcggctgg
     4561 cgcgagcccc tgatgctctt cgtccagatc atcctgatcg acaagaccgg cttccatccg
     4621 agtacgtgct cgctcgatgc gatgtttcgc ttggtggtcg aatgggcagg tagccggatc
     4681 aagcgtatgc agccgccgca ttgcatcagc catgatggat actttctcgg caggagcaag
     4741 gtgagatgac aggagatcct gccccggcac ttcgcccaat agcagccagt cccttcccgc
     4801 ttcagtgaca acgtcgagca cagctgcgca aggaacgccc gtcgtggcca gccacgatag
     4861 ccgcgctgcc tcgtcctgca gttcattcag ggcaccggac aggtcggtct tgacaaaaag
     4921 aaccgggcgc ccctgcgctg acagccggaa cacggcggca tcagagcagc cgattgtctg
     4981 ttgtgcccag tcatagccga atagcctctc cacccaagcg gccggagaac ctgcgtgcaa
     5041 tccatcttgt tcaatcatgc gaaacgatcc agatccggtg cagattattt ggattgagag
     5101 tgaatatgag actctaattg gataccgagg ggaatttatg gaacgtcagt ggagcatttt
     5161 tgacaagaaa tatttgctag ctgatagtga ccttaggcga cttttgaacg cgcaataatg
     5221 gtttctgacg tatgtgctta gctcattaaa ctccagaaac ccgcggctga gtggctcctt
     5281 caacgttgcg gttctgtcag ttccaaacgt aaaacggctt gtcccgcgtc atcggcgggg
     5341 gtcataacgt gactccctta attctccgct catgatgaat tcagaaataa attcagccta
     5401 attcggcgtt aattcagtac attaaaaacg tccgcaatgt gttattaagt tgtctaagcg
     5461 tcaatttgtt tacaccacaa tatatcctgc caccagccag ccaacagctc cccgaccggc
     5521 agctcggcac aaaatcacca ctcgatacag gcagcccatc agtccgggac ggcgtcagcg
     5581 ggagagccgt tgtaaggcgg cagactttgc tcatgttacc gatgctattc ggaagaacgg
     5641 caactaagct gccgggtttg aaacacggat gatctcgcgg agggtagcat gttgattgta
     5701 acgatgacag agcgttgctg cctgtgatca attcgggcac gaacccagtg gacataagcc
     5761 tcgttcggtt cgtaagctgt aatgcaagta gcgtaactgc cgtcacgcaa ctggtccaga
     5821 accttgaccg aacgcagcgg tggtaacggc gcagtggcgg ttttcatggc ttcttgttat
     5881 gacatgtttt tttggggtac agtctatgcc tcgggcatcc aagcagcaag cgcgttacgc
     5941 cgtgggtcga tgtttgatgt tatggagcag caacgatgtt acgcagcagg gcagtcgccc
     6001 taaaacaaag ttaaacatca tgggggaagc ggtgatcgcc gaagtatcga ctcaactatc
     6061 agaggtagtt ggcgtcatcg agcgccatct cgaaccgacg ttgctggccg tacatttgta
     6121 cggctccgca gtggatggcg gcctgaagcc acacagtgat attgatttgc tggttacggt
     6181 gaccgtaagg cttgatgaaa caacgcggcg agctttgatc aacgaccttt tggaaacttc
     6241 ggcttcccct ggagagagcg agattctccg cgctgtagaa gtcaccattg ttgtgcacga
     6301 cgacatcatt ccgtggcgtt atccagctaa gcgcgaactg caatttggag aatggcagcg
     6361 caatgacatt cttgcaggta tcttcgagcc agccacgatc gacattgatc tggctatctt
     6421 gctgacaaaa gcaagagaac atagcgttgc cttggtaggt ccagcggcgg aggaactctt
     6481 tgatccggtt cctgaacagg atctatttga ggcgctaaat gaaaccttaa cgctatggaa
     6541 ctcgccgccc gactgggctg gcgatgagcg aaatgtagtg cttacgttgt cccgcatttg
     6601 gtacagcgca gtaaccggca aaatcgcgcc gaaggatgtc gctgccgact gggcaatgga
     6661 gcgcctgccg gcccagtatc agcccgtcat acttgaagct agacaggctt atcttggaca
     6721 agaagaagat cgcttggcct cgcgcgcaga tcagttggaa gaatttgtcc actacgtgaa
     6781 aggcgagatc accaaggtag tcggcaaata atgtctagct agaaattcgt tcaagccgac
     6841 gccgcttcgc cggcgttaac tcaagcgatt agatgcacta agcacataat tgctcacagc
     6901 caaactatca ggtcaagtct gcttttatta tttttaagcg tgcataataa gccctacaca
     6961 aattgggaga tatatcatgc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
     7021 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
     7081 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
     7141 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
     7201 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
     7261 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
     7321 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
     7381 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
     7441 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
     7501 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
     7561 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
     7621 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
     7681 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
     7741 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
     7801 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
     7861 gcggtatttc acaccgcata tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     7921 taagccagta tacactccgc tatcgctacg tgactgggtc atggctgcgc cccgacaccc
     7981 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
     8041 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
     8101 cgcgaggcag ggtgccttga tgtgggcgcc ggcggtcgag tggcgacggc gcggcttgtc
     8161 cgcgccctgg tagattgcct ggccgtaggc cagccatttt tgagcggcca gcggccgcga
     8221 taggccgacg cgaagcggcg gggcgtaggg agcgcagcga ccgaagggta ggcgcttttt
     8281 gcagctcttc ggctgtgcgc tggccagaca gttatgcaca ggccaggcgg gttttaagag
     8341 ttttaataag ttttaaagag ttttaggcgg aaaaatcgcc ttttttctct tttatatcag
     8401 tcacttacat gtgtgaccgg ttcccaatgt acggctttgg gttcccaatg tacgggttcc
     8461 ggttcccaat gtacggcttt gggttcccaa tgtacgtgct atccacagga aagagacctt
     8521 ttcgaccttt ttcccctgct agggcaattt gccctagcat ctgctccgta cattaggaac
     8581 cggcggatgc ttcgccctcg atcaggttgc ggtagcgcat gactaggatc gggccagcct
     8641 gccccgcctc ctccttcaaa tcgtactccg gcaggtcatt tgacccgatc agcttgcgca
     8701 cggtgaaaca gaacttcttg aactctccgg cgctgccact gcgttcgtag atcgtcttga
     8761 acaaccatct ggcttctgcc ttgcctgcgg cgcggcgtgc caggcggtag agaaaacggc
     8821 cgatgccggg atcgatcaaa aagtaatcgg ggtgaaccgt cagcacgtcc gggttcttgc
     8881 cttctgtgat ctcgcggtac atccaatcag ctagctcgat ctcgatgtac tccggccgcc
     8941 cggtttcgct ctttacgatc ttgtagcggc taatcaaggc ttcaccctcg gataccgtca
     9001 ccaggcggcc gttcttggcc ttcttcgtac gctgcatggc aacgtgcgtg gtgtttaacc
     9061 gaatgcaggt ttctaccagg tcgtctttct gctttccgcc atcggctcgc cggcagaact
     9121 tgagtacgtc cgcaacgtgt ggacggaaca cgcggccggg cttgtctccc ttcccttccc
     9181 ggtatcggtt catggattcg gttagatggg aaaccgccat cagtaccagg tcgtaatccc
     9241 acacactggc catgccggcc ggccctgcgg aaacctctac gtgcccgtct ggaagctcgt
     9301 agcggatcac ctcgccagct cgtcggtcac gcttcgacag acggaaaacg gccacgtcca
     9361 tgatgctgcg actatcgcgg gtgcccacgt catagagcat cggaacgaaa aaatctggtt
     9421 gctcgtcgcc cttgggcggc ttcctaatcg acggcgcacc ggctgccggc ggttgccggg
     9481 attctttgcg gattcgatca gcggccgctt gccacgattc accggggcgt gcttctgcct
     9541 cgatgcgttg ccgctgggcg gcctgcgcgg ccttcaactt ctccaccagg tcatcaccca
     9601 gcgccgcgcc gatttgtacc gggccggatg gtttgcgacc gtcacgccga ttcctcgggc
     9661 ttgggggttc cagtgccatt gcagggccgg cagacaaccc agccgcttac gcctggccaa
     9721 ccgcccgttc ctccacacat ggggcattcc acggcgtcgg tgcctggttg ttcttgattt
     9781 tccatgccgc ctcctttagc cgctaaaatt catctactca tttattcatt tgctcattta
     9841 ctctggtagc tgcgcgatgt attcagatag cagctcggta atggtcttgc cttggcgtac
     9901 cgcgtacatc ttcagcttgg tgtgatcctc cgccggcaac tgaaagttga cccgcttcat
     9961 ggctggcgtg tctgccaggc tggccaacgt tgcagccttg ctgctgcgtg cgctcggacg
    10021 gccggcactt agcgtgtttg tgcttttgct cattttctct ttacctcatt aactcaaatg
    10081 agttttgatt taatttcagc ggccagcgcc tggacctcgc gggcagcgtc gccctcgggt
    10141 tctgattcaa gaacggttgt gccggcggcg gcagtgcctg ggtagctcac gcgctgcgtg
    10201 atacgggact caagaatggg cagctcgtac ccggccagcg cctcggcaac ctcaccgccg
    10261 atgcgcgtgc ctttgatcgc ccgcgacacg acaaaggccg cttgtagcct tccatccgtg
    10321 acctcaatgc gctgcttaac cagctccacc aggtcggcgg tggcccatat gtcgtaaggg
    10381 cttggctgca ccggaatcag cacgaagtcg gctgccttga tcgcggacac agccaagtcc
    10441 gccgcctggg gcgctccgtc gatcactacg aagtcgcgcc ggccgatggc cttcacgtcg
    10501 cggtcaatcg tcgggcggtc gatgccgaca acggttagcg gttgatcttc ccgcacggcc
    10561 gcccaatcgc gggcactgcc ctggggatcg gaatcgacta acagaacatc ggccccggcg
    10621 agttgcaggg cgcgggctag atgggttgcg atggtcgtct tgcctgaccc gcctttctgg
    10681 ttaagtacag cgataacctt catgcgttcc ccttgcgtat ttgtttattt actcatcgca
    10741 tcatatacgc agcgaccgca tgacgcaagc tgttttactc aaatacacat caccttttta
    10801 gacggcggcg ctcggtttct tcagcggcca agctggccgg ccaggccgcc agcttggcat
    10861 cagacaaacc ggccaggatt tcatgcagcc gcacggttga gacgtgcgcg ggcggctcga
    10921 acacgtaccc ggccgcgatc atctccgcct cgatctcttc ggtaatgaaa aacggttcgt
    10981 cctggccgtc ctggtgcggt ttcatgcttg ttcctcttgg cgttcattct cggcggccgc
    11041 cagggcgtcg gcctcggtca atgcgtcctc acggaaggca ccgcgccgcc tggcctcggt
    11101 gggcgtcact tcctcgctgc gctcaagtgc gcggtacagg gtcgagcgat gcacgccaag
    11161 cagtgcagcc gcctctttca cggtgcggcc ttcctggtcg atcagctcgc gggcgtgcgc
    11221 gatctgtgcc ggggtgaggg tagggcgggg gccaaacttc acgcctcggg ccttggcggc
    11281 ctcgcgcccg ctccgggtgc ggtcgatgat tagggaacgc tcgaactcgg caatgccggc
    11341 gaacacggtc aacaccatgc ggccggccgg cgtggtggtg tcggcccacg gctctgccag
    11401 gctacgcagg cccgcgccgg cctcctggat gcgctcggca atgtccagta ggtcgcgggt
    11461 gctgcgggcc aggcggtcta gcctggtcac tgtcacaacg tcgccagggc gtaggtggtc
    11521 aagcatcctg gccagctccg ggcggtcgcg cctggtgccg gtgatcttct cggaaaacag
    11581 cttggtgcag ccggccgcgt gcagttcggc ccgttggttg gtcaagtcct ggtcgtcggt
    11641 gctgacgcgg gcatagccca gcaggccagc ggcggcgctc ttgttcatgg cgtaatgtct
    11701 ccggttctag tcgcaagtat tctactttat gcgactaaaa cacgcgacaa gaaaacgcca
    11761 ggaaaagggc agggcggcag cctgtcgcgt aacttaggac ttgtgcgaca tgtcgttttc
    11821 agaagacggc tgcactgaac gtcagaagcc gactgcacta tagcagcgga ggggttggat
    11881 caaagtact
//
